Macrophage Activation and Polarization

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Lu, C., Kang, T., Zhang, J., Yang, K., Liu, Y., Song, K., Lin, Q., Dixit, D., Gimple, R. C., Zhang, Q., Shi, Z., Fan, X., Wu, Q., Li, D., Shan, D., Gao, J., Gu, D., You, H., Li, Y., … Wang, X. (2025). Combined targeting of glioblastoma stem cells of different cellular states disrupts malignant progression. Nature Communications, 16(1). https://doi.org/10.1038/s41467-025-58366-5
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Maurer, K., Park, C. Y., Mani, S., Borji, M., Raths, F., Gouin, K. H., Penter, L., Jin, Y., Zhang, J. Y., Shin, C., Brenner, J. R., Southard, J., Krishna, S., Lu, W., Lyu, H., Abbondanza, D., Mangum, C., Olsen, L. R., Lawson, M. J., … Azizi, E. (2025). Coordinated immune networks in leukemia bone marrow microenvironments distinguish response to cellular therapy. Science Immunology, 10(103). https://doi.org/10.1126/sciimmunol.adr0782
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Chatila, Z. K., Mares, J., Vardarajan, B. N., Elyaman, W., Menon, V., & Bradshaw, E. M. (2024). Microglial Activation Alters Chromatin Accessibility of AD‐Associated Transcription Factors. Alzheimer’s & Dementia, 20(S1). Portico. https://doi.org/10.1002/alz.085990
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Green, J. L., Dressman, D., Rashid, M., Hobson, R., Elyaman, W., & Bradshaw, E. M. (2024). CD33 competitive inhibitor peptide IMAD‐001 modulates innate immunity in Alzheimer’s disease. Alzheimer’s & Dementia, 20(S6). Portico. https://doi.org/10.1002/alz.089331
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Wu, X., Wang, Z., Shern, T., & Zhang, H. (2024). Efferocytosis assay to quantify the engulfment and acidification of apoptotic cells by macrophages using flow cytometry. STAR Protocols, 5(3), 103215. https://doi.org/10.1016/j.xpro.2024.103215
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Nguyen, T. T. T., Gao, Q., Mun, J.-Y., Zhu, Z., Shu, C., Naim, A., Rogava, M., Izar, B., Westhoff, M.-A., Karpel-Massler, G., & Siegelin, M. D. (2024). Suppressing PD-L1 Expression via AURKA Kinase Inhibition Enhances Natural Killer Cell-Mediated Cytotoxicity against Glioblastoma. Cells, 13(13), 1155. https://doi.org/10.3390/cells13131155
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Haq, I., Ngo, J. C., Roy, N., Pan, R. L., Nawsheen, N., Chiu, R., Zhang, Y., Fujita, M., Soni, R. K., Wu, X., Bennett, D. A., Menon, V., Olah, M., & Sher, F. (2024). An integrated toolkit for human microglia functional genomics. Stem Cell Research & Therapy, 15(1). https://doi.org/10.1186/s13287-024-03700-9
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He, S., Jin, Y., Nazaret, A., Shi, L., Chen, X., Rampersaud, S., Dhillon, B. S., Valdez, I., Friend, L. E., Fan, J. L., Park, C. Y., Mintz, R. L., Lao, Y.-H., Carrera, D., Fang, K. W., Mehdi, K., Rohde, M., McFaline-Figueroa, J. L., Blei, D., … Azizi, E. (2024). Starfysh integrates spatial transcriptomic and histologic data to reveal heterogeneous tumor–immune hubs. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02173-8
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Qian, J., Ryeom, S., Daugherty, B. L., Lederman, S., & Wang, T. C. (2023). 481 A CXCR4 partial agonist TFF2-MSA sensitized advanced gastric cancer to PD-1 blockade by systematically reducing PMN-MDSC accumulation, immunosuppression, and generation in bone marrow. Regular and Young Investigator Award Abstracts. https://doi.org/10.1136/jitc-2023-sitc2023.0481
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Daugherty, B. L., Boohaker, R. J., Johnstone, R., Stinson, K., Zhao, G., Zang, M., Qian, J., Wang, T. C., & Lederman, S. (2023). 1345 mTFF2-MSA (mTNX-1700) suppresses tumor growth and increases survival in anti-PD-1 treated CT26.wt subcutaneous and CT26-Luciferase orthotopic syngeneic colorectal cancer models by targeting MDSCs. Regular and Young Investigator Award Abstracts. https://doi.org/10.1136/jitc-2023-sitc2023.1345
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Patel, S. P., Carvajal, R., Montazeri, K., Reddy, S., Lutzky, J., Khan, S. A., Chmielowski, B., Bhatia, S., Medina, T., Haymaker, C., Sheth, R., Kuban, J. D., Thornton, L., Wehrenberg-Klee, E., Novelli, P., Lucci, A., Sarli, V., Meas, S., Cox, B., … Davar, D. (2023). 1534 Clinical activity of SD-101 with immune checkpoint inhibition (ICI) in metastatic uveal melanoma liver metastasis (MUM-LM) from the PERIO-01 Phase 1 trial. Late-Breaking Abstracts. https://doi.org/10.1136/jitc-2023-sitc2023.1534
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Sanchini, C., Rosito, M., Comincini, A., De Panfilis, S., Bartolini, F., & Di Angelantonio, S. (2023). Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells. STAR Protocols, 4(3), 102499. https://doi.org/10.1016/j.xpro.2023.102499
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Chen, L., Shi, V., Wang, S., Sun, L., Freeman, R., Yang, J., Inkman, M. J., Ghosh, S., Ruiz, F., Jayachandran, K., Huang, Y., Luo, J., Zhang, J., Cosper, P., Luke, C. J., Spina, C. S., Grigsby, P. W., Schwarz, J. K., & Markovina, S. (2023). SCCA1/SERPINB3 suppresses antitumor immunity and blunts therapy-induced T cell responses via STAT-dependent chemokine production. Journal of Clinical Investigation, 133(15). https://doi.org/10.1172/jci163841
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Hobson, B. D., Stanley, A. T., De Los Santos, M. B., Culbertson, B., Mosharov, E. V., Sims, P. A., & Sulzer, D. (2023). Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain. Brain, Behavior, and Immunity, 111, 277–291. https://doi.org/10.1016/j.bbi.2023.04.008
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Laternser, S., Petrovic, A., Eze, A., Dettwiler, S., Prutek, F., Contini, P. A., Morinigo, D., Bornhorst, M., Rushing, E. J., McDonough, E., Surrette, C., Ginty, F., Minnis, H., Gartrell, R., Müller, S., Packer, R., & Nazarian, J. (2023). DIPG-25. MAPPING TUMOR MICROENVIRONMENT USING EMERGING TECHNOLOGIES FOR PEDIATRIC BRAIN CANCERS. Neuro-Oncology, 25(Supplement_1), i18–i18. https://doi.org/10.1093/neuonc/noad073.072
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Shin, A. E., Tesfagiorgis, Y., Larsen, F., Derouet, M., Zeng, P. Y. F., Good, H. J., Zhang, L., Rubinstein, M. R., Han, Y. W., Kerfoot, S. M., Nichols, A. C., Hayakawa, Y., Howlett, C. J., Wang, T. C., & Asfaha, S. (2023). F4/80+Ly6Chigh Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology, 164(4), 593-609.e13. https://doi.org/10.1053/j.gastro.2023.01.002
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Mannes, P. Z., Barnes, C. E., Biermann, J., Latoche, J. D., Day, K. E., Zhu, Q., Tabary, M., Xiong, Z., Nedrow, J. R., Izar, B., Anderson, C. J., Villanueva, F. S., Lee, J. S., & Tavakoli, S. (2023). Molecular imaging of chemokine-like receptor 1 (CMKLR1) in experimental acute lung injury. Proceedings of the National Academy of Sciences, 120(3). https://doi.org/10.1073/pnas.2216458120
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Tanagala, K. K. K., Morin-Baxter, J., Carvajal, R., Cheema, M., Dubey, S., Nakagawa, H., Yoon, A., Cheng, Y.-S. L., Taylor, A., Nickerson, J., Mintz, A., & Momen-Heravi, F. (2022). SP140 inhibits STAT1 signaling, induces IFN-γ in tumor-associated macrophages, and is a predictive biomarker of immunotherapy response. Journal for ImmunoTherapy of Cancer, 10(12), e005088. https://doi.org/10.1136/jitc-2022-005088
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Zhou, R. W., Xu, J., Martin, T. C., Zachem, A. L., He, J., Ozturk, S., Demircioglu, D., Bansal, A., Trotta, A. P., Giotti, B., Gryder, B., Shen, Y., Wu, X., Carcamo, S., Bosch, K., Hopkins, B., Tsankov, A., Steinhagen, R., Jones, D. R., … Parsons, R. E. (2022). A local tumor microenvironment acquired super-enhancer induces an oncogenic driver in colorectal carcinoma. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-33377-8
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Gupta, P., Dang, M., Hao, D., Bojja, K., Tran, T., Shehwana, H., Kamiya-Matsuoka, C., Li, J., Audia, A., Kassab, C., Ott, M., Gumin, J., Alenazy, S., Goldman, A., Seth, S., Maheshwari, A., Balasubramaniyan, V., Vaillant, B., Groot, J. de, … Bhat, K. (2022). 1001 Immunophenotyping of brain tumors identifies interferon enriched phagocytes in human gliomas. Regular and Young Investigator Award Abstracts, A1043–A1043. https://doi.org/10.1136/jitc-2022-sitc2022.1001
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Peereboom, D., Lindsay, R., Badruddoja, M., Nabors, L. B., Kumthekar, P., Lieberman, F., Tran, D., Phuphanich, S., Schiff, D., Sherman, J., Butowski, N., Dunbar, E., Fink, K., Iwamoto, F., Moertel, C., Schulder, M., Walbert, T., Habboubi, N., Grzegorzewski, K., … Reardon, D. A. (2022). CTIM-29. PHASE 2 STUDY OF A NOVEL IMMUNOTHERAPY SL-701 IN ADULTS WITH RECURRENT GBM: IDENTIFICATION OF TREATMENT-INDUCED CD8+CD107A+ CD57+ PD-1- MEMORY T-CELLS THAT ARE ASSOCIATED WITH INCREASED SURVIVAL. Neuro-Oncology, 24(Supplement_7), vii67–vii67. https://doi.org/10.1093/neuonc/noac209.261
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Alvarez-Breckenridge, C., Markson, S. C., Stocking, J. H., Nayyar, N., Lastrapes, M., Strickland, M. R., Kim, A. E., de Sauvage, M., Dahal, A., Larson, J. M., Mora, J. L., Navia, A. W., Klein, R. H., Kuter, B. M., Gill, C. M., Bertalan, M., Shaw, B., Kaplan, A., Subramanian, M., … Carter, S. L. (2022). Microenvironmental Landscape of Human Melanoma Brain Metastases in Response to Immune Checkpoint Inhibition. Cancer Immunology Research, 10(8), 996–1012. https://doi.org/10.1158/2326-6066.cir-21-0870
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Schilperoort, M., Ngai, D., & Tabas, I. (2022). Efferocytosis induces a transient burst of glycolysis in macrophages to promote lactate-driven binding and continuing removal of apoptotic cells. Atherosclerosis, 355, 1–2. https://doi.org/10.1016/j.atherosclerosis.2022.06.091
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Henick, B. S., Villarroel-Espindola, F., Datar, I., Sanmamed, M. F., Yu, J., Desai, S., Li, A., Aguirre-Ducler, A., Syrigos, K., Rimm, D. L., Chen, L., Herbst, R. S., & Schalper, K. A. (2022). Quantitative tissue analysis and role of myeloid cells in non-small cell lung cancer. Journal for ImmunoTherapy of Cancer, 10(7), e005025. https://doi.org/10.1136/jitc-2022-005025
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Rouanne, M., Adam, J., Radulescu, C., Letourneur, D., Bredel, D., Mouraud, S., Goubet, A.-G., Leduc, M., Chen, N., Tan, T. Z., Signolle, N., Bigorgne, A., Dussiot, M., Tselikas, L., Susini, S., Danlos, F.-X., Schneider, A. K., Chabanon, R., Vacher, S., … Marabelle, A. (2022). BCG therapy downregulates HLA-I on malignant cells to subvert antitumor immune responses in bladder cancer. Journal of Clinical Investigation, 132(12). https://doi.org/10.1172/jci145666
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Minns, H. E., Padilla, O., Wei, H.-J., Webster-Carrion, A., Tazhibi, M., McQuillan, N., Zhang, X., Yeh, R., Zhang, Z., Szalontay, L., Pavisic, J., Garty, G., Garvin, J., Zacharoulis, S., Canoll, P., Vanpouille-Box, C. I., Menon, V., Olah, M., Rabadan, R., … Gartrell, R. D. (2022). DIPG-45. Radiation induces a robust interferon response in Diffuse Midline Glioma (DMG), improving the potential for combination immunotherapy. Neuro-Oncology, 24(Supplement_1), i28–i29. https://doi.org/10.1093/neuonc/noac079.102
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Mysore, V., Tahir, S., Furuhashi, K., Arora, J., Rosetti, F., Cullere, X., Yazbeck, P., Sekulic, M., Lemieux, M. E., Raychaudhuri, S., Horwitz, B. H., & Mayadas, T. N. (2022). Monocytes transition to macrophages within the inflamed vasculature via monocyte CCR2 and endothelial TNFR2. Journal of Experimental Medicine, 219(5). https://doi.org/10.1084/jem.20210562
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Galán-Díez, M., Borot, F., Ali, A. M., Zhao, J., Gil-Iturbe, E., Shan, X., Luo, N., Liu, Y., Huang, X.-P., Bisikirska, B., Labella, R., Kurland, I., Roth, B. L., Quick, M., Mukherjee, S., Rabadán, R., Carroll, M., Raza, A., & Kousteni, S. (2022). Subversion of Serotonin Receptor Signaling in Osteoblasts by Kynurenine Drives Acute Myeloid Leukemia. Cancer Discovery, 12(4), 1106–1127. https://doi.org/10.1158/2159-8290.cd-21-0692
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Sahm, V., Maurer, C., Baumeister, T., Anand, A., Strangmann, J., Schmid, R. M., Wang, T. C., & Quante, M. (2022). Telomere shortening accelerates tumor initiation in the L2-IL1B mouse model of Barrett esophagus and emerges as a possible biomarker. Oncotarget, 13(1), 347–359. https://doi.org/10.18632/oncotarget.28198
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Cohn, W., Melnik, M., Huang, C., Teter, B., Chandra, S., Zhu, C., McIntire, L. B., John, V., Gylys, K. H., & Bilousova, T. (2021). Multi-Omics Analysis of Microglial Extracellular Vesicles From Human Alzheimer’s Disease Brain Tissue Reveals Disease-Associated Signatures. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.766082
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